Apolipoprotein E genotyping using the polymerase chain reaction and allele-specific oligonucleotide primers.
نویسندگان
چکیده
منابع مشابه
Apolipoprotein E genotyping using the polymerase chain reaction and allele-specific oligonucleotide probes.
A rapid procedure for determining apolipoprotein E genotype from genomic DNA has been developed. In this procedure, DNA is amplified by the polymerase chain reaction, and allele-specific oligonucleotide probes are used to detect the cysteine-arginine interchanges at residues 112 and 158 that distinguish the three common isoforms of apolipoprotein E. The method was tested with 68 subjects, repre...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1991
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)42257-6